Journal: Oncotarget

Article Title: New strategy to rescue the inhibition of osteogenesis of human bone marrow-derived mesenchymal stem cells under oxidative stress: combination of vitamin C and graphene foams

doi: 10.18632/oncotarget.12456

Figure Lengend Snippet: ( A ) Representative image of BM-MSCs 5 days after seeding. ( B – D ) Characterization of BM-MSCs by flow cytometry. The majority of the cells are CD105 + , CD44 + and CD29 + , which are typical characteristic phenotypes of BM-MSCs. ( E ) BM-MSC can be differentiated into adipogenic and myogenic lineages. Adipogenic differentiation was characterized by Oil Red O staining, while myogenic differentiation was evidenced by the formation of myotubes stained with crystal violet. ( F ) Representative image of immunostaining of BM-MSCs on GF scaffold, stained by anti-β-tubulin (green) and DAPI for nucleus (blue). Effects of different concentrations of VC (5 to 100 μg/ml, 5 days) ( G ) and H 2 O 2 exposure (0.1 to 2 mM, 24 hours) ( H ) on cell viability of BM-MSCs cultured for 5 days, measured by MTT assay. ( I ) Cell viability of BM-MSCs in the five experimental groups. Data were presented as mean ± SEM. * p < 0.05 vs control.

Article Snippet: Antibodies used in the study were as follows: CD105-FITC (MCA1557FT, Serotec, Oxford, UK), CD44-FITC (MCA643FA, Serotec), and CD29-FITC (MCA1949FT, Serotec).

Techniques: Flow Cytometry, Staining, Immunostaining, Cell Culture, MTT Assay, Control

Journal: Clinical Cancer Research

Article Title: Mesenchymal Stem Cell Targeting of Microscopic Tumors and Tumor Stroma Development Monitored by Noninvasive In vivo Positron Emission Tomography Imaging

doi: 10.1158/1078-0432.ccr-05-0876

Figure Lengend Snippet: Fig. 5. In situ characterization of EGFP+ cells in HT-29 (s.c.) + TG-hMSC (i.v.) tumors. A, agarose gel electrophoresis of PCR-amplified EGFP sequence (717 bp) done using1 Ag of cellular DNA prepared from loosely associated cell fraction (loose), stromal matrix associated fraction (stroma), and the residue (residue) of HT-29 (s.c.)/TG-hMSC (i.v.) tumor and HT-29 (s.c.) tumor,TG-hMSC, and HT-29 cells. B, cytofluorometric histograms comparing the stromal matrix cell fractions of HT-29 (s.c.) + TG-hMSC (i.v.) tumors (red) and HT-29 (s.c.) tumors (black), examined with anti-GFP specific antibody. EGFP+ cells in the gated area represented 11.5% of total cells. C, histograms showing the presence of human endothelial cell markers but absence of hMSC markers in EGFP+ cells obtained from the HT-29 (s.c.) + TG-hMSC (i.v.) tumor after 30 days of growth in vivo. Stromal matrix ^ associated cell fractions were double stained with FITC-labeled anti-GFP mouse monoclonal antibody, and with a PE-labeled anti-vWF, or anti-CD31, or anti-CD90, or anti-CD105 to assess the expression of these cell surface markers on EGFP+ cells (red). PE-labeled mouse isotype immunoglobulins were included in parallel to serve as the negative control (black).

Article Snippet: © 2005 American Association for Cancerclincancerres.aacrjournals.org Downloaded from anti-mouse IgG antibody or PE-labeled anti-hvWF; and PE-labeled mouse monoclonal antibodies against human CD14 (PharMingen, San Diego, CA), CD31, CD90, CD105 (Serotec, Raleigh, NC), and smooth muscle actin (Chemicon, Temecula, CA).

Techniques: In Situ, Agarose Gel Electrophoresis, Amplification, Sequencing, Residue, In Vivo, Staining, Labeling, Expressing, Negative Control

Journal: Apoptosis

Article Title: Adipose stromal/stem cells assist fat transplantation reducing necrosis and increasing graft performance

doi: 10.1007/s10495-013-0878-7

Figure Lengend Snippet: Antibodies for rabbit ASC characterization by FACS

Article Snippet: ASC (1 × 10 6 ) were labelled with the following monoclonal antibodies (Table ): FITC conjugated anti-rat CD90 (Biolegend, Milan, Italy), unconjugated anti-rat CD45 (Genetex, Milan, Italy), unconjugated anti-human CD29, CD49e, and CD105 (all from Serotec, Milan, Italy), APC conjugated anti-human CD10 and PE conjugated anti-human CD73 (BD) unconjugated anti-rabbit CD31 and CD146 (Abcam, Cambridge, UK).

Techniques: